Steven Gygi

  • Latest publications

    Wuhr, M. et al. The Nuclear Proteome of a Vertebrate. Curr Biol 25, 2663-2671 (2015)

    Paulo, J.A., O’Connell, J.D., Gaun, A. & Gygi, S.P. Proteome-wide quantitative multiplexed profiling of protein expression: carbon-source dependency in Saccharomyces cerevisiae. Mol Biol Cell 26, 4063-4074 (2015).

    Huttlin, E.L. et al. The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 162, 425-440 (2015).

    Chick, J.M. et al. A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides. Nat Biotechnol 33, 743-749 (2015).

    Weekes, M.P. et al. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157, 1460-1472 (2014).

  • Prizes and Awards

    Armenise-Harvard Junior Faculty Grant, Department of   Integrative Biology & Physiology: “Global Protein Expression Profiling”, 2000

Who he is

Steven Gygi received his Ph.D. from the University of Utah in Pharmacology and Toxicology. His focus was on small molecule detection by mass spectrometry. He next moved to Seattle for a postdoctoral fellowship with Ruedi Aebersold at the University of Washington. In 2000, Steve started his own lab at Harvard Medical School. His current rank is Professor in the Department of Cell Biology.

What he does

Research in the Gygi lab centers on the proteome-wide measurement and characterization of proteins and posttranslational modifications by high performance mass spectrometry. One of the most dramatic developments in biological research is the shift from the analysis of single genes and proteins to the comprehensive analysis of biological systems and pathways. This shift is a consequence of the development of automated, high-throughput genomic technologies and their application to sequence complete genomes and to measure gene expression on a genome-wide scale.

Currently, comparably powerful technologies are becoming available for the analysis of biological systems on the protein level. Proteins are the functional units of the living world—the molecules which carry out most catalytic, structural, and regulatory functions inside cells. A complete model of a biological process cannot be established without knowledge of the involved players and mechanistic characterization of their functional roles. The lab is  committed to the development and application of technologies for the rapid, sensitive, quantitative and multiplexed analysis of protein expression and protein phosphorylation profiles from diverse cellular settings.

News from the Lab

The Gygi lab in collaboration with Wade Harper’s lab recently published the BioPlex network. BioPlex is a protein interaction network that is made up of more than 2,500 different immunoprecipitation experiments with different human proteins as baits. The network spans more than 9,000 proteins and is the largest of its kind.