Angela DePace

  • Latest publications

    Wunderlich Z, Bragdon MD, Vincent BJ, White JA, Estrada J, DePace AH. (2015). Krüppel Expression Levels Are Maintained through Compensatory Evolution of Shadow Enhancers. Cell Rep. pii: S2211-1247(15)00892-X. PMID: 26344774.

    Staller MV, Fowlkes CC, Bragdon MD, Wunderlich Z, Estrada J, DePace AH. (2015). A gene expression atlas of a bicoid-depleted Drosophila embryo reveals early canalization of cell fate. Development, 142(3):587-96. PMID: 25605785. PMCID: PMC4302997.

    Staller MV, Vincent BJ, Bragdon MD, Lydiard-Martin T, Wunderlich Z, Estrada J, DePace AH. (2015). Shadow enhancers enable Hunchback bifunctionality in the Drosophila embryo. Proc Natl Acad Sci U S A., 112(3):785-90. PMID: 25564665. PMCID: PMC4311800.

    Ilsley G.R., Fisher J., Apweiler R., DePace A.H., Luscombe N.M. (2013). Cellular resolution models for even skipped regulation in the entire Drosophila embryo. Elife, 2:e00522. PMID: 23930223. PMCID: PMC3736529.

    Wunderlich, Z., Bragdon, M.D., Eckenrode, K.B., Lydiard-Martin, T., Pearl-Waserman, S., DePace, A.H. (2012). Dissecting sources of quantitative gene expression pattern divergence between Drosophila species. Mol Syst Biol., 8:604. PMID: 22893002. PMCID: PMC3435502.

  • Prizes and Awards

    NSF CAREER Award, 2015

    Everett Mendelsohn Excellence in Mentoring Award, 2012

    Armenise-Harvard Junior Faculty Grant, Department of Systems Biology: “Cis-regulatory control of quantitative variation in gene expression in Drosophila”, 2009

Who she is

Angela obtained her B.S. in Molecular Biochemistry and Biophysics at  Yale, and her PhD in Biochemistry at the University of California San Francisco, where she worked with Jonathan Weissman on the mechanism of prion propagation. In 2008, Angela began her lab in the Systems Biology Department at Harvard Medical School with the help of a Junior Faculty grant from the Armenise Foundation.

 

What she does

Regulation of gene expression is critical for every biological process, from development and differentiation to aging and disease. In contrast to sequences coding for proteins, we cannot yet “read” gene regulation directly from any genome because we do not have a clear mechanistic picture of transcription. The goal of Angela’s lab is to understand, in biophysical terms, how genomic sequences integrate information to produce gene expression patterns. Deciphering these fundamental molecular mechanisms will have widespread consequences for how we identify regulatory DNA and how we interpret regulatory DNA sequence, two challenges that are central to achieving precision medicine.

News from the Lab

The lab is currently investigating two fundamental aspects of regulatory DNA function. First, researchers are probing the extent to which enhancers, which direct expression patterns in space and time, behave as independent modules. Their data indicate that enhancer function is sensitive to context, which has important consequences for how to interpret regulatory sequence variants such as duplications, deletions, and translocations. Second, Angela’s group is developing new experimental and theoretical approaches for probing the dynamic role of transcription factors in orchestrating the transcriptional cycle, including non-equilibrium processes such as nucleosome remodeling and post-translational modification.